Sarojini Enterprises
Sarojini Enterprises
Hyderguda, Hyderabad, Telangana
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Hepatitis B Test Kit

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Qualisa Microwell Enzyme Immunoassay Hepatitis B Test Kit

Qualisa Microwell Enzyme Immunoassay Hepatitis B Test Kit
  • Qualisa Microwell Enzyme Immunoassay Hepatitis B Test Kit
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Approx. Price: Rs 1,450 / BoxGet Latest Price

Product Details:

Minimum Order Quantity5 Box
Sensitivity100%
PrincipleEnzyme Linked Immunosorbent Assay (ELISA)
ApplicationFor detection of Hepatitis B surface antigen in human serum/plasma
Pack Size96 test
Pack TypeBox
Storage2-8 degree C, 12 months

Test Procedure:
  • Bring all the reagents and specimen to room temperature before use.
  • Take out required number of strips and immediately close the pouch.
  • Prepare ELISA protocol sheet indicating the location of controls and specimen.
  • Use controls in duplicate.
  • Add 50 μl activated conjugate to all the wells.
  • Add 100 μl undiluted control or specimen in separate wells. Mix by gentle tapping.
  • Apply plate sealer and incubate according to the procedure chosen.
  • Wash each well by filling 350 μl diluted wash buffer and aspirating / flicking off six times with 1 minute soak time between
  • each wash. Blot dry.
ProcedureIncubation Time
Short Procedure60 min. at 37°C
Standard Procedure120 min. at 37°C
Overnight ProcedureOvernight at 22-28°C

  • Add 100μl substrate and incubate at 22-28°C away from light for 30 minutes.
  • Stop reaction by adding 100μl stop solution. The stop solution should be added in the same sequence as substrate
  • addition.
  • Read the absorbance at 450 nm with 600-700 nm as reference within 30 minutes of stopping the reaction.

 

Reagents:

  • Coated microwells: Microwells coated with agglutinating sera for HBsAg. Ready to use.
  •  Conjugate: Agglutinating sera for HBsAg- HRPO. (Horse Radish Peroxidase). Ready to use.
  •  Conjugate Activator: Buffered solution containing activator and preservatives
  •  Substrate: Solution containing Tetramethyl benzidine (TMB) and hydrogen peroxide. Ready to use.
  •  Wash buffer: Buffer contains surfactants
    (20 X). To be diluted 20 times with
    distilled or deionized water.
  •  Stop solution: Diluted acid. Ready to use.
  •  Positive Control: HBsAg positive and HIV/ HCV non-reactive serum diluted in stabilizer solution with preservatives.
  •  Negative control: Inactivated and stabilized human serum non-reactive for HIV-1 and HIV-2, HCV & HBsAg.
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B Srinivas (Owner)
G-23, & 24, Ground Floor, Agarwal Chambers
Hyderguda, Hyderabad- 500001, Telangana, India



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