Hepatitis B Test Kit

Product Details:

• Bring all the reagents and specimen to room temperature before use.
• Take out required number of strips and immediately close the pouch.
• Prepare ELISA protocol sheet indicating the location of controls and specimen.
• Use controls in duplicate.
• Add 50 μl activated conjugate to all the wells.
• Add 100 μl undiluted control or specimen in separate wells. Mix by gentle tapping.
• Apply plate sealer and incubate according to the procedure chosen.
• Wash each well by filling 350 μl diluted wash buffer and aspirating / flicking off six times with 1 minute soak time between
• each wash. Blot dry.

• Add 100μl substrate and incubate at 22-28°C away from light for 30 minutes.
• Stop reaction by adding 100μl stop solution. The stop solution should be added in the same sequence as substrate
• addition.
• Read the absorbance at 450 nm with 600-700 nm as reference within 30 minutes of stopping the reaction.

Reagents:

• Coated microwells: Microwells coated with agglutinating sera for HBsAg. Ready to use.
•  Conjugate: Agglutinating sera for HBsAg- HRPO. (Horse Radish Peroxidase). Ready to use.
•  Conjugate Activator: Buffered solution containing activator and preservatives
•  Substrate: Solution containing Tetramethyl benzidine (TMB) and hydrogen peroxide. Ready to use.
•  Wash buffer: Buffer contains surfactants
  (20 X). To be diluted 20 times with
  distilled or deionized water.
•  Stop solution: Diluted acid. Ready to use.
•  Positive Control: HBsAg positive and HIV/ HCV non-reactive serum diluted in stabilizer solution with preservatives.
•  Negative control: Inactivated and stabilized human serum non-reactive for HIV-1 and HIV-2, HCV & HBsAg.