HIV Test Kit

Product Details:

• Bring all the reagents and specimen to room temperature before use.
• Take out required number of strips and immediately close the pouch.
• Prepare ELISA protocol sheet indicating the location of controls and specimen.
• Use controls in duplicate.
• Add 200 µl sample diluent to separate wells.
• Add 10 µl of control or specimen in the wells.
• Gently shake the plate to mix thoroughly. Care should be taken to avoid spillage.
• Apply plate sealer and incubate for 30 minutes at 22-28 C.
• Wash each well by filling approximately 350 µl diluted wash buffer and aspirating/flicking off six times. Blot dry.
• Add 100 µl diluted conjugate in each well and incubate for 30 minutes at 22-28 C.
• Wash six times as in step 9. Blot dry.
• Add 100 µl substrate and incubate at 22-28 C away from light for 30 minutes.
• Stop reaction by adding 100 µl stop solution. The stop solution should be added in the same sequence as substrate addition.
• Read the absorbance at 450 nm with 600-700 nm as reference within 30 minutes of stopping the reaction.

• Coated microwells:96 microwells (3x8 wells x 4) are coated with monoclonal anti p-24 antibodies and recombinant antigens representing both HIV1 and HIV2
• Positive control : Inactivated and stabilized human serum reactive for HIV1 with preservatives
• Negative control : Inactivated and stabilized human serum non reactive for HIV-1and HIV-2, HBsAg and HCV
• Conjugate: Antigen-HRP conjugate (50 x). To be diluted 50 times with conjugate diluent.
• Antibody Reagent: Biotinylated monoclonal anti-p24 antibody. Ready to use.
• Conjugate diluent : Antigen-HRP conjugate (50x). To be diluted 50 times with conjugate diluent.
• Activator: Buffered solution containing activator and preservatives.
• Substrate: Solution containing (TMB) and hydrogen peroxide. Ready to use.
• Wash buffer : Buffer containing surfactants (20x). To be diluted 20 times with distilled water or deionized water.
• Stop solution : Dilute Sulphuric acid.

Product Details:

• Bring all the reagents and specimen to room temperature before use.
• Take out required number of strips and immediately close the pouch.
• Prepare data sheet indicating the location of controls and specimen.
• Use controls in duplicate.
• Add 50 µl activated antibody reagent in each well .
• Add 100 µl control or specimen in separate wells.
• Apply plate sealer and incubate for 60 minutes at 37 C .
• Wash each well by filling approximately 350µl diluted wash buffer, giving 30 seconds soak time for each wash and aspirating/flicking off six times. Blot dry.
• Add 100 µl diluted conjugate in each well and incubate for 30 minutes at 37 C.
• Wash each well by filling approximately 350µl diluted wash buffer, giving 30 seconds soak time for each and aspirating/flicking off six times. Blot dry.
• Add 100µl substrate in each well and incubate for 30 minutes at room temperature (22-28 C) away from direct light.
• Stop reaction by adding 100µl stop solution. The stop solution should be added in the same sequence as substrate addition.
• Read the absorbance at 450 nm with 600-700 nm as reference within 30 minutes of stopping the reaction

• High p24 antigen sensitivity: 20 pg/ml
• High S/CO
• DHS technology
• High specificity
• Early detection within 2 weeks

• Excellent detectability and specificity
• Higher OD values in low titre samples and ''grayzone'' reactions are highly in true positive samples
• Good correlation with other licensed ELISAs
• High detectability particularly in low titre samples
• High antibody sensitivity
• High thermal stability
• Long-use life Serves to contain the spread of infection, by timely detection

Product Details:

• Bring the sealed aluminium foil pouch ofQUALPRO HIV membrane test assembly to room temperature.
• Open a foil pouch by tearing along the "notch".
• Remove the membrane test assembly and the sample applicator. Once opened, the membrane test assembly must be used immediately.
• Label the membrane test assembly with specimen identity.
• Place the membrane test assembly on a flat horizontal surface.
• Carefully dispense one drop (25µl) of serum / plasma into the specimen well ‘S’using the sample applicator provided.
• Add three drops of sample running buffer into the same well ‘S’.
• Observe the development of visible coloured band at Test regions (‘1’for HIV-1 and/or ‘2’for HIV-2).
• Positive results may be observed within 20 minutes.
• The test should be considered invalid if the control band ‘C’ does not appear. The test is also invalid if neither the control
• TM nor the test bands appear. Repeat the test with a new QUALPRO HIV membrane test assembly.