Sarojini Enterprises
Sarojini Enterprises
Hyderguda, Hyderabad, Telangana
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HIV Test Kit

Wholesale Trader of a wide range of products which include qualisa microwell enzyme immunoassay hiv test kit, qualisa hiv 4.0 test kit and qualpro hiv test kit.

Qualisa Microwell Enzyme Immunoassay HIV Test Kit

Qualisa Microwell Enzyme Immunoassay HIV Test Kit
  • Qualisa Microwell Enzyme Immunoassay HIV Test Kit
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Approx. Price: Rs 3,500 / BoxGet Latest Price

Product Details:

Minimum Order Quantity5 Box
Usage/ApplicationFor detection of antibodies to HIV V1/2 & O subtypes and p24 antigen in human serum or plasma.
Sample TypePlasma, Serum
Sensitivity100%
Temperature Storage2-8 degree C, 12 months
PrincipleEnzyme linked immunoassay (ELISA)
Test Duration180 minutes
Pack Size96 test
Pack TypeBox
BrandQualisa
TypeHIV 1 & 2
Specificity99.7%

Being one of the renowned organizations in the industry, we are highly engaged in providing a premium quality range of Qualisa Microwell Enzyme Immunoassay HIV Test Kit.

Test Procedure:
  • Bring all the reagents and specimen to room temperature before use.
  • Take out required number of strips and immediately close the pouch.
  • Prepare ELISA protocol sheet indicating the location of controls and specimen.
  • Use controls in duplicate.
  • Add 200 µl sample diluent to separate wells.
  • Add 10 µl of control or specimen in the wells.
  • Gently shake the plate to mix thoroughly. Care should be taken to avoid spillage.
  • Apply plate sealer and incubate for 30 minutes at 22-28 C.
  • Wash each well by filling approximately 350 µl diluted wash buffer and aspirating/flicking off six times. Blot dry.
  • Add 100 µl diluted conjugate in each well and incubate for 30 minutes at 22-28 C.
  • Wash six times as in step 9. Blot dry.
  • Add 100 µl substrate and incubate at 22-28 C away from light for 30 minutes.
  • Stop reaction by adding 100 µl stop solution. The stop solution should be added in the same sequence as substrate addition.
  • Read the absorbance at 450 nm with 600-700 nm as reference within 30 minutes of stopping the reaction.

Reagents:
  • Coated microwells:96 microwells (3x8 wells x 4) are coated with monoclonal anti p-24 antibodies and recombinant antigens representing both HIV1 and HIV2
  • Positive control : Inactivated and stabilized human serum reactive for HIV1 with preservatives
  • Negative control : Inactivated and stabilized human serum non reactive for HIV-1and HIV-2, HBsAg and HCV
  • Conjugate: Antigen-HRP conjugate (50 x). To be diluted 50 times with conjugate diluent.
  • Antibody Reagent: Biotinylated monoclonal anti-p24 antibody. Ready to use.
  • Conjugate diluent : Antigen-HRP conjugate (50x). To be diluted 50 times with conjugate diluent.
  • Activator: Buffered solution containing activator and preservatives.
  • Substrate: Solution containing (TMB) and hydrogen peroxide. Ready to use.
  • Wash buffer : Buffer containing surfactants (20x). To be diluted 20 times with distilled water or deionized water.
  • Stop solution : Dilute Sulphuric acid.
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Qualisa HIV 4.0 Test Kit

Qualisa HIV 4.0 Test Kit
  • Qualisa HIV 4.0 Test Kit
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Approx. Price: Rs 3,500 / BoxGet Latest Price

Product Details:

Minimum Order Quantity5 Box
Sample Volume30 ul blood
Result Time1 min
Sensitivity100%
Storage2 - 8 Deg C, Do Not Freeze
Tests Kit96 Number
Specificity99.71%

We are successfully engaged in providing a wide gamut of Qualisa HIV 4.0 Test Kit.

Test Procedure:
  • Bring all the reagents and specimen to room temperature before use.
  • Take out required number of strips and immediately close the pouch.
  • Prepare data sheet indicating the location of controls and specimen.
  • Use controls in duplicate.
  • Add 50 µl activated antibody reagent in each well .
  • Add 100 µl control or specimen in separate wells.
  • Apply plate sealer and incubate for 60 minutes at 37 C .
  • Wash each well by filling approximately 350µl diluted wash buffer, giving 30 seconds soak time for each wash and aspirating/flicking off six times. Blot dry.
  • Add 100 µl diluted conjugate in each well and incubate for 30 minutes at 37 C.
  • Wash each well by filling approximately 350µl diluted wash buffer, giving 30 seconds soak time for each and aspirating/flicking off six times. Blot dry.
  • Add 100µl substrate in each well and incubate for 30 minutes at room temperature (22-28 C) away from direct light.
  • Stop reaction by adding 100µl stop solution. The stop solution should be added in the same sequence as substrate addition.
  • Read the absorbance at 450 nm with 600-700 nm as reference within 30 minutes of stopping the reaction

Features:
  • High p24 antigen sensitivity: 20 pg/ml
  • High S/CO
  • DHS technology
  • High specificity
  • Early detection within 2 weeks

Benefits:
  • Excellent detectability and specificity
  • Higher OD values in low titre samples and 'grayzone' reactions are highly in true positive samples
  • Good correlation with other licensed ELISAs
  • High detectability particularly in low titre samples
  • High antibody sensitivity
  • High thermal stability
  • Long-use life Serves to contain the spread of infection, by timely detection
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Qualpro HIV Test Kit

Qualpro HIV Test Kit
  • Qualpro HIV Test Kit
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Approx. Price: Rs 5,000 / BoxGet Latest Price

Product Details:

Minimum Order Quantity10 Box
Pack Size25 test
Pack TypeBox
Storage Temperature4 degree to 30 degree celsius, Do not freeze
Sample Applicators25 x Disposable Sample Applicators
Package Insert1 x Package Insert

With our in-depth knowledge of this domain, we are actively engaged in providing an excellent quality range of Qualpro HIV Test Kit.

Test Procedure:
  • Bring the sealed aluminium foil pouch ofQUALPRO HIV membrane test assembly to room temperature.
  • Open a foil pouch by tearing along the "notch".
  • Remove the membrane test assembly and the sample applicator. Once opened, the membrane test assembly must be used immediately.
  • Label the membrane test assembly with specimen identity.
  • Place the membrane test assembly on a flat horizontal surface.
  • Carefully dispense one drop (25µl) of serum / plasma into the specimen well ‘S’using the sample applicator provided.
  • Add three drops of sample running buffer into the same well ‘S’.
  • Observe the development of visible coloured band at Test regions (‘1’for HIV-1 and/or ‘2’for HIV-2).
  • Positive results may be observed within 20 minutes.
  • The test should be considered invalid if the control band ‘C’ does not appear. The test is also invalid if neither the control
  • TM nor the test bands appear. Repeat the test with a new QUALPRO HIV membrane test assembly.
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B Srinivas (Owner)
G-23, & 24, Ground Floor, Agarwal Chambers
Hyderguda, Hyderabad- 500001, Telangana, India



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